Essay about Micro Lab Project - 757 Words

Quantitative Semi-quantitative Viable Plate Counts Molly Wright, Jenny Cano, Rosa Ramirez BIOL 2420 Lab M/W 4/20/15 Professor Rotibi ABSTRACT: Accurate evaluation of bacterial colonization as a predictive index for alfalfa sprouts has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of the alfalfa water. In this study 1 package of alfalfa sprouts were cultured by a semi-quantitative technique that enumerated the number of gram-negative enteric organism in 1 ml of alfalfa water. Exact colony counts from the experiment were available only from cultures 10-2 and 10-3 CFUs. Other colony counts were reported as less than 10-3 or greater than†¦show more content†¦Preparing serial dilutions of the alfalfa water will ensure an accurate count. The plates are incubated until you see visible colonies, usually 18-24 hours. The colonies you see growing on the plate are considered to have started from one viable bacterial unit but because bacteria are usually not found as individuals, the colony you see may have started from a single cel l or a group of cells. The results are reported as colony forming units (CFU’s). Materials: -1 pkg. of alfalfa sprouts purchased at a local grocery store or farmer’s market, submerged in 100 ml of sterile, distilled water. (Any other food or beverage item may be substituted for alfalfa sprouts) -Graduated pipettes (sterile) -Pipettor -5 tubes containing 9 ml of sterile water -5 MacConkey Agar plates -Glass hockey stick -Jar of alcohol -Bunsen burner -Flint striker -Microscope slide Glass coverslips Method: 1. Make a wet mount (Ex 12) of a small sample of the alfalfa water. Examine at 1000X total magnification using high contrast. This will illustrate how many organisms, both motile and non-motile, are present in the sample. 2. 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